Involvement of alpha-1-adrenergic receptors in central region of amygdala and the effects of cannabinoid agonist on inhibitory avoidance memory in male rats

There are many similarities between memory impairment in patients suffering from Alzheimer and animals treated by Cannabinoids. The agonists of Cannabinoid receptors affect on a variety of memories and leanings. The present study aims to investigate the role of alpha-1-adrenergic receptors in central region of amygdala in state-dependent learning induced by WIN55,212-2 [cannabinoid agonist] in rats. Cannulae placement was performed bilaterally in the central amygdala region of male rats. The rats were trained in the avoidance learning apparatus [step-down model]. 24 hours after training, the memory was tested by measuring the lag time for stepping down the platform. Injection of WIN55, 212-2 intra central amygdala [dose-dependent, 0.25, 0.5 micro g/rat] post-training reduced lag time/latency for stepping down. Injection induced amnesia was reversed by pre-test administration of the same dose of WIN55, 212-2. It is called state-dependent learning. Pre-test intra-central injection of alpha-1-adrenoceptor agonist, Phenylephrine [0.5, 0.25 micro g/rat] improved post-training WIN55, 212-2 [0.5 micro g/rat] intra central injection induced retrieval impairment. But intra injection of Prazosin [0.5 micro g/rat] 2 minutes before injection of WIN55, 212-2 [0.5 micro g/rat] on the testing day inhibited WIN55, 212-2 state-dependent learning. The results suggest that alpha-1-adrenergic receptors in central region of amygdala are involved in learning which dependent on the state induced by WIN55, 212-2

[ effect of dorsal hippocampal alpha2-adrenegic receptors on WIN55,212-2 state-dependent memory of passive avoidance]

Cannabinoids are a class of psychoactive compounds that produce a wide array of effects in a large number of species. In the present study, the effects of bilateral intra-CA1 injections of an alpha2-adrenergic receptor agents, on WIN55, 212-2 state-dependent learning were examined in adult male Wistar rats. The animals were bilaterally implanted with chronic cannulae in the CA1 regions of the dorsal hippocampus, trained in a step-down type inhibitory avoidance task, and tested 24h after training to measure step-down latency. Post-training intra-CA1 injection of WIN55, 212-2 [0.25 and 0.5microg/rat] induced impairment of memory retention. Amnesia produced by post-training WIN55, 212-2 [0.5microg/rat] was reversed by pre-test administration of the same dose of WIN55, 212-2 that is due to a state-dependent effect. Pre-test intra-CA1 injection of clonidine [0.5 and 0.75microg/rat, intra-CA1] improved post-training WIN55, 212-2 [0.5microg/rat, intra-CA1]-induced retrieval impairment, while pre-test intra-CA1 injection of yohimbine [1microg/rat, intra-CA1] 2min before the administration of WIN55, 212-2 [0.5microg/rat, intra-CA1] inhibited WIN55, 212-2 state-dependent memory. These results suggest that alpha2-adrenergic receptors of the dorsal hippocampal CA1 regions may play an important role in Win55, 212-2-induced amnesia and WIN55, 212-2 state-dependent memory

Thesynthesis and antimicrobil properties of 4-chloro-2, 6-bis [2-hydroxy - tolyl] phenol

The synthesis of the title compound is described. This compound was found to be active [3c] against a number of pathogenic microorganisms in vitro. It is a non-absorbable antibacterial topically and its pharmacologic studies revealed that it is a non-toxic agent with a wide range of safety. It proved to be effective in the prevention and treatment of pseudomonas wound infections in volunteer patients